phospho protein expression (LI-COR)
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phospho protein expression
Phospho Protein Expression, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 198 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/celltag+700+stain/CELLTAG+700+STAIN/pmc13051198-66-6-15
Average 98 stars, based on 198 article reviews
Phospho Protein Expression, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 198 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/celltag+700+stain/CELLTAG+700+STAIN/pmc13051198-66-6-15
Average 98 stars, based on 198 article reviews
phospho protein expression - by Bioz Stars,
2026-09
98/100 stars
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Staining:Article Title: The effect of macromolecular crowders as a supplement to serum free media on human corneal stromal cells proliferation and marker expression Article Snippet: Fluorescence reading of the fluorometer (Thermo Fisher Scientific) with excitation at 530 nm and emission at 590 nm was recorded for each culture condition. .. The final number of viable cells was evaluated after 21 days by Article Title: The effect of macromolecular crowders as a supplement to serum free media on human corneal stromal cells proliferation and marker expression. Article Snippet: Fluorescence reading of the fluorometer (Thermo Fisher Scientific) with excitation at 530nm and emission at 590nm was recorded for each culture condition. .. Cell Tag staining: The final number of viable cells was evaluated after 21 days by Article Title: Ketoconazole-carbonic anhydrase inhibitors as potential anticancer agents. Article Snippet: The biochemical complexity of cancers offers the possibility to repurpose known drugs for new therapeutic applications.. In this study, we exploited the potential anticancer effects of a series of compounds structurally organized with the tumor-associated Carbonic Anhydrase (CA; EC 4.2.1.1) IX/XII inhibiting warhead and the antifungal drug Ketoconazole (KTZ), which was recently considered in oncology for its ability to suppress steroidogenesis as well as disruption of the biogenesis of extracellular vesicles (EVs).. The data here reported gives the first line of evidence that the in vitro antiproliferative effects of such compounds on a panel of tumoral cell lines are ascribed to integration of the anti-CA IX/XII effect with the EV pathway disruption and thus might represent a promising dual-mechanism therapeutic approach. Article Title: Leveraging Viscosity to Unlock the Osteogenic Potential of BMP-2 Mimetic DWIVA. Article Snippet: Primary antibodies used were anti-RUNX2 (1:100, mouse monoclonal; Santa Cruz Biotechnology sc-390351), anti-phospho-RUNX2 (1:250, rabbit polyclonal; Invitrogen PA5105642), anti-phospho SMAD1/5/9 (1:800, rabbitmonoclonal; Cell Signaling #13820), anti-phospho-ERK1/2 (1:200, rabbit monoclonal; Cell Signaling #4370), anti-phospho-p38 (1:800, rabbit monoclonal; Cell Signaling #4511), and were added overnight at 4◦C. .. Secondary antibodies used were IRDye 800CW Goat anti-rabbit (1:800, Li-cor 926-32211), IRDye 800CW Donkey antimouse (1:800, Li-cor 926-32212), IRDye 680RD Donkey antimouse (1:800; Li-cor 926-68072), Article Title: Leveraging Viscosity to Unlock the Osteogenic Potential of BMP‐2 Mimetic DWIVA Article Snippet: Primary antibodies used were anti‐RUNX2 (1:100, mouse monoclonal; Santa Cruz Biotechnology sc‐390351), anti‐phospho‐RUNX2 (1:250, rabbit polyclonal; Invitrogen PA5‐105642), anti‐phospho SMAD1/5/9 (1:800, rabbit monoclonal; Cell Signaling #13820), anti‐phospho‐ERK1/2 (1:200, rabbit monoclonal; Cell Signaling #4370), anti‐phospho‐p38 (1:800, rabbit monoclonal; Cell Signaling #4511), and were added overnight at 4°C. .. Secondary antibodies used were IRDye 800CW Goat anti‐rabbit (1:800, Li‐cor 926‐32211), IRDye 800CW Donkey anti‐mouse (1:800, Li‐cor 926‐32212), IRDye 680RD Donkey anti‐mouse (1:800; Li‐cor 926‐68072), Article Title: NaBC1 Acts as a Mechanosensitive Co-regulator of Fibronectin-binding Integrins Adhesion and Myoblast Polarization Article Snippet: Cells were then incubated with primary antibodies: anti-NaBC1 (ABCAM, 1:200), anti-integrin α 5 (ABCAM, 1:500), anti-integrin α v (ABCAM, 1:500) and anti-Akt/pAkt (Invitrogen, 1:500) diluted in blocking buffer overnight at 4°C. .. After 3 washes of 10 minutes with 0.1% PBST buffer, cells were incubated with 1:800 diluted infrared-labeled secondary antibody IRDye 800CW (LI-COR) and 1:500 diluted Article Title: Targeting leukemic stem and progenitor cells expressing different BCR::ABL1 levels: antileukemic activity of asciminib with or without TKIs Article Snippet: .. Relative quantification of both total and phospho-protein expression was normalized using the Control:Article Title: Ketoconazole-carbonic anhydrase inhibitors as potential anticancer agents. Article Snippet: The biochemical complexity of cancers offers the possibility to repurpose known drugs for new therapeutic applications.. In this study, we exploited the potential anticancer effects of a series of compounds structurally organized with the tumor-associated Carbonic Anhydrase (CA; EC 4.2.1.1) IX/XII inhibiting warhead and the antifungal drug Ketoconazole (KTZ), which was recently considered in oncology for its ability to suppress steroidogenesis as well as disruption of the biogenesis of extracellular vesicles (EVs).. The data here reported gives the first line of evidence that the in vitro antiproliferative effects of such compounds on a panel of tumoral cell lines are ascribed to integration of the anti-CA IX/XII effect with the EV pathway disruption and thus might represent a promising dual-mechanism therapeutic approach. Incubation:Article Title: NaBC1 Acts as a Mechanosensitive Co-regulator of Fibronectin-binding Integrins Adhesion and Myoblast Polarization Article Snippet: Cells were then incubated with primary antibodies: anti-NaBC1 (ABCAM, 1:200), anti-integrin α 5 (ABCAM, 1:500), anti-integrin α v (ABCAM, 1:500) and anti-Akt/pAkt (Invitrogen, 1:500) diluted in blocking buffer overnight at 4°C. .. After 3 washes of 10 minutes with 0.1% PBST buffer, cells were incubated with 1:800 diluted infrared-labeled secondary antibody IRDye 800CW (LI-COR) and 1:500 diluted Quantitative Proteomics:Article Title: Targeting leukemic stem and progenitor cells expressing different BCR::ABL1 levels: antileukemic activity of asciminib with or without TKIs Article Snippet: .. Relative quantification of both total and phospho-protein expression was normalized using the Expressing:Article Title: Targeting leukemic stem and progenitor cells expressing different BCR::ABL1 levels: antileukemic activity of asciminib with or without TKIs Article Snippet: .. Relative quantification of both total and phospho-protein expression was normalized using the |